The cAMP-induced G protein subunits dissociation monitored in live Dictyostelium cells by BRET reveals two activation rates, a positive effect of caffeine and potential role of microtubules. Journal of Allergy and Clinical Immunology. From these experiments, a nonchloro TOM (NCT) ligand was identified as providing the greatest signal over the background. does not suffer from high fluorescent background or photobleaching, the common problems assocd. A Novel Luminescence-Based High-Throughput Approach for Cellular Resolution of Protein Ubiquitination Using Tandem Ubiquitin Binding Entities (TUBEs). Daniel Jiménez Fernández, Sandra Hess, Klaus-Peter Knobeloch. Advances in Cellular Target Engagement and Target Deconvolution.
Hyun Yong Jin, Yanyan Tudor, Kaylee Choi, Zhifei Shao, Brian A. Sparling, Joseph G. McGivern, Antony Symons. stability, retaining activity with incubation up to 55 °C or in culture medium for >15 h at 37 °C. TurboYFP is a fast maturing YFP variant derived from, Having established HaloTag-NCT as an optimal energy acceptor for Nluc, this combination should enhance the performance of BRET for analyzing intracellular protein–protein interactions (PPI).
Wampler J E; Hori K; Lee J W; Cormier M J. Fractional occupancy (%) indicates the amount of acceptor-occupied Nluc-HT relative to the total amount of Nluc-HT in the sample.
Two emitters during both the in vitro and the in vivo bioluminescence of the sea pansy, Renilla, https://chemport.cas.org/services/resolver?origin=ACS&resolution=options&coi=1%3ACAS%3A280%3ADyaE38%252FivVersA%253D%253D&md5=5d8c04a505737bcb83b9a6b468849dca, Illuminating insights into protein-protein interactions using bioluminescence resonance energy transfer (BRET), https://chemport.cas.org/services/resolver?origin=ACS&resolution=options&coi=1%3ACAS%3A528%3ADC%252BD28XhsFOru7k%253D&md5=bc83e4f90f5ab10ed12286eb20849ccc, The BRET2/arrestin assay in stable recombinant cells: a platform to screen for compounds that interact with G protein-coupled receptors (GPCRS), Journal of Receptors and Signal Transduction, https://chemport.cas.org/services/resolver?origin=ACS&resolution=options&coi=1%3ACAS%3A528%3ADC%252BD38Xps1ejsbY%253D&md5=a2b21a28d1737f18c064989299e33086, High-throughput screening of G protein-coupled receptor antagonists using a bioluminescence resonance energy transfer 1-based 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Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. A polymorphic residue that attenuates the antiviral potential of interferon lambda 4 in hominid lineages. These data establish proof-of-concept for targeting protein-protein interactions of epigenetic 'readers', and provide a versatile chem.
Mutational analysis of the extracellular disulphide bridges of the atypical chemokine receptor ACKR3/CXCR7 uncovers multiple binding and activation modes for its chemokine and endogenous non-chemokine agonists. proteins, functions exclusively to recognize acetyl-lysine on histones and non-histone proteins.
Sandra Röhm, Benedict-Tilman Berger, Martin Schröder, Apirat Chaikuad, Rob Winkel, Koen F. W. Hekking, Jorg J. C. Benningshof, Gerhard Müller, Roberta Tesch, Mark Kudolo, Michael Forster, Stefan Laufer.
Prolyl Isomerase Pin1 Regulates the Stability of Hepatitis B Virus Core Protein.
Makoto Habara, Nobuko Mori, Yuki Okada, Koh Kawasumi, Nobuhiro Nakao, Yoshikazu Tanaka, Toshiro Arai, Ichiro Yamamoto. The American Chemical Society holds a copyright ownership interest in any copyrightable Supporting Eline Pottie, Dilip K. Tosh, Zhan-Guo Gao, Kenneth A. Jacobson, Christophe P. Stove.
The Angiotensin Receptor Blocker Losartan Suppresses Growth of Pulmonary Metastases via AT1R-Independent Inhibition of CCR2 Signaling and Monocyte Recruitment. Anum A. Glasgow, Yao-Ming Huang, Daniel J. Mandell, Michael Thompson, Ryan Ritterson, Amanda L. Loshbaugh, Jenna Pellegrino, Cody Krivacic, Roland A. Pache, Kyle A. Barlow, Noah Ollikainen, Deborah Jeon, Mark J. S. Kelly, James S. Fraser, Tanja Kortemme. Thus, a series of stable compositions can be readily generated that hold the total amount of Nluc constant while accurately varying the portion occupied by an acceptor. Link between a high 2 This is clearly demonstrated in our examples using NanoBRET to monitor interactions with chromatin. A Chemical Probe for Tudor Domain Protein Spindlin1 to Investigate Chromatin Function. Progress in the Development of non-BET Bromodomain Chemical Probes. Quantitative Live-Cell Kinetic Degradation and Mechanistic Profiling of PROTAC Mode of Action. Among Aequorea GFP variants, yellow fluorescent proteins (YFPs) are relatively acid-sensitive, and uniquely quenched by chloride ion (Cl-). Tobias Wimmer, Eva Schroeter, Birgit Lorenz, Knut Stieger. Scott A. Busby, Seth Carbonneau, John Concannon, Christoph E. Dumelin, YounKyoung Lee, Shin Numao, Nicole Renaud, Thomas M. Smith. Zinaida M. Kaskova, Aleksandra S. Tsarkova, Ilia V. Yampolsky. Furthermore, for the 1st time it was possible to use the BRET2 system to detect ligand-induced G protein-coupled receptor/β-arrestin interactions over prolonged periods (on the scale of hours rather than seconds) with a very stable signal.
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