In addition, the routine buffers provided with typical Taq-based products from numerous vendors are also expected to be compatible with this product. Figure 3. Original Research on Thermo Fisher Scientific Molecular ... It not only significantly reduces errors in the barcoding and amplification steps, but also captures twice more UMIs in the library than . Invitrogen™ Platinum™ SuperFi™ DNA Polymerase is a proofreading DNA polymerase which combines superior fidelity with trusted PlatinumTM hot-start technology for the highest success in PCR. This protocol minimizes sample loss and significantly reduces protocol time with no DNA purification or cell lysis steps. polymerase). Platinum™ SuperFi™ DNA Polymerase: 17500円（キャンペーンなし？） 175円（キャンペーンなし？） ThermoScientific: AccuPrime™ Pfx DNA Polymerase: 65900円（キャンペーンなし？） 329.5円（キャンペーンなし？ The Exo-CIP Rapid PCR Cleanup Kit has been tested for compatibility with the following master mixes and buffers. 6 Template plasmid DNA (250 pg/µl) 4 μl 25 pg/µl 5x SuperFi™ Buffer 8 μl 1x 5x SuperFi™ GC Enhancer 8 μl 1x 10 mM dNTP mix 0.8 μl 0.2 mM 100 nM gene specific primer 4 μl 10 nM 100 nM antisense primer 4 μl 10 nM Platinum™ SuperFi™ DNA Polymerase 0.4 μl (*1) 0.02 U/μl Total 40 μl polymerase promoter and a dedicated translation initiation site (E01 translational enhancer) that supports Cap-independent initiation of translation. $206.00 / Each. Platinum SuperFi PCR Master Mix. Enzyme: Platinum™ SuperFi™ DNA Polymerase Activities: 5' to 3' polymerase, 3' to 5' exonuclease (proofreading) Hot-start: Antibody Fidelity vs. Taq: >100X PCR setup Component 50-µL rxn Final conc. Learn more about the use of different PCR enzymes for cloning application by visiting PCR educational . 100 Rxn 12532016. 12344024. Platinum SuperFi DNA Polymerase is a proofreading DNA polymerase that combines superior fidelity with reliable Platinum Hot-Start technology for highest success in PCR. #12351-050) Thermo Scientific 10 mM dNTP Mixes (Cat. If interference is observed (due to . AccuPrime™ Pfx DNA Polymerase. Platinum SuperFi PCR Master Mix, 100rxn. Search online for Assays, Antibodies, Oligos; pH and Electrochemistry. Catalog No. Invitrogen™ Platinum™ SuperFi™ DNA Polymerase is a proofreading DNA polymerase that combines exceptional fidelity with trusted Platinum™ hot-start technology, offering the highest success in PCR. Amplify even difficult and GC-rich amplicons with superior accuracy (>100X Taq) and minimaloptimization steps. Incubate at RT on a rotator for 1 h. Wash the beads 5x using 0.05% PBST and 3x using 1 x PBS, for a total of eight washes. 100 Rxn . SKU: 12358010 Category: Thermo Fisher / Invitrogen. The parameters were adjusted on a set of primers seeking to maximize specificity and retain high yield with Platinum SuperFi DNA Polymerase.The modified Breslauer's thermodynamics method (2) is is used for Tm and annealing temperature calculation of reactions with Phusion or Phire DNA Polymerases.A separate method is used for T m and annealing . SKU: 12358010 Category: Thermo Fisher / Invitrogen. Invitrogen™ Platinum™ SuperFi™ DNA Polymerase is a proofreading DNA polymerase that combines superior fidelity with trusted Platinum™ hot-start technology for the highest success in PCR. Platinum SuperFi DNA Polymerase: Phusion Hot Start II High-Fidelity DNA Polymerase: Phusion U Hot Start DNA Polymerase: Phusion High-Fidelity DNA Polymerase: Fidelity vs. Taq polymerase >100x: 52x: 25x: 52x: Hot-start PCR: Antibody-based: Affibody-based: Affibody-based: No: Target length: ≤20 kb: ≤20 kb: ≤20 kb: ≤20 kb: Extension rate . Invitrogen™ Platinum™ SuperFi II DNA Polymerase is a proofreading DNA polymerase that combines superior fidelity with an innovative buffer, enabling universal primer annealing for the highest success in PCR. Effect of polymerase fidelity in the adapter PCR. Taq (7). Taq. EA. Platinum™ PCR SuperMix, High Fidelity. Pricing and Availability. 1, 3, 7, 10, and 13 kb targets from Lambda genomic DNA were amplified following manufacturer's standards for PCR cycling parameters. View more versions of this product. 12346086. Add to cart. Being an open in vitro translation system, proteins can be expressed from linear DNA templates as well as using an expression vector encoding the Open Reading Frame (ORF) for the protein of interest. The average stutter values at the loci D5S818, D21S11, and D18S51 were 5.56%, 7.47%, and 6.53% for AmpliTaq Gold 360, but 0.86%, 1.64%, and 5.18% for SuperFi, respectively. Featuring >100x Taq fidelity, Platinum SuperFi DNA Polymerase is ideally suited for cloning, mutagenesis and other applications benefiting from supreme . Determination of SiMSen-Seq sensitivity. Proprietary additives in the . Browse through our "Guides and Tools" section to access a comprehensive portfolio of product-related support resources. With >300x Taq fidelity and a specially formulated buffer for primer annealing at 60°C, Thermo Fisher Scientific Invitrogen™ Pla SuperFi GC Enhancer was added for 70% and 76% GC fragments. Molecular Biology Product Discontinuations. Briefly, the PCR cycle is as follows: Initialization step. I have used Tm Calculator (Thermo Fisher Scientific) to measure the Tm of the first set of primers (without . Higher PCR yield: 47%. Platinum SuperFi PCR Master Mix, 100rxn. Featuring >100x Taq fidelity, Platinum SuperFi DNA Polymerase is ideally suited for cloning, mutagenesis and other applications benefiting from supreme . AccuPrime™ Pfx DNA Polymerase. Each. Description. If interference is observed (due to . NTC=no template control. Add to cart. Abstract: Platinum ™ SuperFi ™ DNA Polymerase is a new proofreading DNA polymerase, which combines superior fidelity with Platinum ™ hot-start technology.Featuring >100X Taq fidelity, Platinum SuperFi DNA Polymerase is ideally suited for cloning, mutagenesis, template generation for sequencing and other applications requiring high . Thermo Scientific™ Phusion™ High-Fidelity DNA Polymerase (2 U/µL) Get superior performance for cloning and other applications requiring high fidelity with one of the most accurate thermostable DNA polymerases available. Manufacturer: Invitrogen™ 12351010. The SuperScript IV One-Step RT-PCR System with The Invitrogen™ SuperScript™ IV One-Step RT-PCR System with ezDNase combines the high processivity Invitrogen™ SuperScript™ IV Reverse Transcriptase (RT) and the high-fidelity Invitrogen™ Platinum™ SuperFi™ DNA Polymerase to provide unmatched one-step RT-PCR performance. Platinum SuperFi PCR Master Mix quantity. 11708013. Robust amplification of challenging templates (e.g., GC-rich, low-purity, degraded) 57%. Featuring greater than 100x Taq fidelity, Platinum SuperFi DNA Polymerase Platinum SuperFi PCR Master Mix quantity. Description. Description. Platinum SuperFi PCR Master Mix retains . 117 Phusion® High-Fidelity DNA Polymerase (New England Biolabs, NEB Cat# M0530), Q5® 118 High-Fidelity DNA Polymerases (NEB, Cat# M0491), Platinum™ SuperFi™ DNA Polymerase 119 (Invitrogen, Cat# 12351010), PrimeSTAR GXL DNA Polymerase (Takara Bio, Cat# R050Q) 120 and Taq polymerase (home-made). TA cloning is also possible if 3′ dA-overhangs are added after PCR. Invitrogen Platinum SuperFi DNA Polymerase is a proofreading DNA polymerase that combines superior fidelity with trusted Platinum hot-start technology for the highest success in PCR. Invitrogen™ Platinum™ SuperFi™ PCR Master Mix is a ready-to-use mixture of DNA polymerase, salts, magnesium, and dNTPs for efficient PCR amplification. This step heats the solutions to 94-98°C for DNA polymerase . Explore our "Getting Started" and "Troubleshooting" sections for solutions to top inquiries and common problems.
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